The limited success of somatic cell nuclear transfer (SCNT) is largely attributed to defects in epigenetic reprogramming of the donor genome.
Donor cell types with distinct potential competence may offer different epigenetic flexibility for subsequent genome reprogramming in SCNT.
To improve the efficiency of cloning, porcine mesenchymal stem cells (pMSCs) were isolated and well identified by 6-channel flow cytometry and differentiation assays and were used as donors in SCNT. The results suggest that the epigenetic status of donor cells has an improvement on genome reprogramming, and multipotent pMSCs favored subsequent embryonic development.
— From Porcine Mesenchymal Stem Cells: Establishment, Differentiation, Electroporation and Nuclear Transfer, by Song, Z., Cong, P., Ji, Q., Chen, L., Nie, Y., Zhao, H., He, Z. and Chen, Y. (2015). Reproduction in Domestic Animals, 50: 840-848. doi: 10.1111/rda. 12577
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